Overview of System Components - Revision history

DAharoni at 19:50, 12 January 2016

2016-01-12T19:50:54Z

2016-01-09T22:51:00Z

2016-01-05T23:14:01Z

2016-01-04T22:31:11Z

2016-01-04T22:30:16Z

2015-11-05T22:06:09Z

2015-11-05T22:05:34Z

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[[File:Overview_System.png|400px]]

← Older revision		Revision as of 19:50, 12 January 2016
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	The depth of imaging is limited to around 200um due to the nature of wide-field fluorescence imaging. As the imaging depth increases, the excitation light intensity decreases due to absorption of the above tissue while the scattering of emission light increase. This becomes more of an issue when imaging regions where labeled cells are overlapping along the z-axis.		The depth of imaging is limited to around 200um due to the nature of wide-field fluorescence imaging. As the imaging depth increases, the excitation light intensity decreases due to absorption of the above tissue while the scattering of emission light increase. This becomes more of an issue when imaging regions where labeled cells are overlapping along the z-axis.
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		+	'''Using this system we have successfully imaged Hippocampal CA1, Subiculum, and Visual Cortex using 2mm diameter GRIN lenses from Grintech.''' While thinner GRIN lenses should theoretically be compatible with our system we have limited our initial development to larger lenses due to supply and experimental constraints.

	*Specifications		*Specifications

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 ** Resolution: 752px x 480px
 ** Super Speed USB: 6Gb/s
 Details on specific components of the system can be found at the links below

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-[[File:Overview_System.png|700px]]
+[[File:Overview_System.png|thumb|600px]]
-[[Head Mounted Scope]]
+Our miniature fluorescence microscope system consists of a lightweight microscope body, optical components, imaging sensor, and read out electronics. The readout electronics handle all communication between the onboard microscope electronics and the host computer such as LED intensity, imaging sensor configuration, and imaging sensor data. The body is machined out of 3 pieces of black Delrin plastic and assembled using self-threading, 1mm diameter screws. The optical components collimate the excitation light, band-pass filter the excitation and emission light, and focus the emission image onto the imaging sensor.
-[[Data Acquisition Box]]
+The depth of imaging is limited to around 200um due to the nature of wide-field fluorescence imaging. As the imaging depth increases, the excitation light intensity decreases due to absorption of the above tissue while the scattering of emission light increase. This becomes more of an issue when imaging regions where labeled cells are overlapping along the z-axis.
-[[Data Acquisition Software]]
+*Specifications