Surgery Protocol - Revision history

Tristanshuman: /* Relay Baseplating */

2019-10-17T14:55:32Z

‎Relay Baseplating

2019-10-17T14:53:02Z

‎Relay Baseplating

2019-10-17T14:51:13Z

‎Baseplating Protocol

2016-11-16T01:04:25Z

‎Baseplating

2016-11-16T01:03:30Z

‎Baseplating

2016-11-16T01:02:51Z

‎Baseplate Preparation

2016-11-16T01:02:31Z

‎Baseplate Preparation

2016-09-21T04:15:15Z

‎Basic Equipment needed

2016-09-21T04:12:53Z

‎Basic Equipment needed

2016-09-21T04:12:39Z

‎Baseplate Preparation

@@ Line 81: / Line 81: @@
 === Relay Baseplating ===
-If using a relay lens for deep imaging, you have 2 options for baseplating. First, you can secure the large objective GRIN lens into the Miniscope (see [[Imaging With Thin GRIN Lenses]]) and baseplate as you would with a single lens (see video above). Alternatively, you can cement that objective lens onto the animals head in order to make it more stable across imaging sessions. We have found this to be far better for stable recording across weeks. The video below steps through this option and demonstrates how to secure the objective GRIN lens into place while imaging.
+If using a relay lens for deep imaging, you have 2 options for baseplating. First, you can secure the large objective GRIN lens into the Miniscope (see [[Imaging With Thin GRIN Lenses]]) and baseplate as you would with a single lens (see video above). If you use this method, you must make the objective lens very secure (with optical or Krazy glue) as any movement of that lens will change the focal plane of the image. Alternatively, you can cement that objective lens onto the animals head in order to make it more stable across imaging sessions. We have found this to be far better for stable recording of the same cells across weeks. The video below steps through this option and demonstrates how to secure the objective GRIN lens into place while imaging.
 {{#ev:youtube|https://youtu.be/b-LrnOBX3j8|640|center}}

@@ Line 81: / Line 81: @@
 === Relay Baseplating ===
-If using a relay lens for deep imaging, you have 2 options for baseplating. First, you can secure the large objective GRIN lens into the Miniscope and baseplate as you would with a single lens (see video above). Alternatively, you can cement that objective lens onto the animals head in order to make it more stable across imaging sessions. We have found this to be far better for stable recording across weeks. The video below steps through this option and demonstrates how to secure the objective GRIN lens into place while imaging.
+If using a relay lens for deep imaging, you have 2 options for baseplating. First, you can secure the large objective GRIN lens into the Miniscope (see [[Imaging With Thin GRIN Lenses]]) and baseplate as you would with a single lens (see video above). Alternatively, you can cement that objective lens onto the animals head in order to make it more stable across imaging sessions. We have found this to be far better for stable recording across weeks. The video below steps through this option and demonstrates how to secure the objective GRIN lens into place while imaging.
 {{#ev:youtube|https://youtu.be/b-LrnOBX3j8|640|center}}

← Older revision		Revision as of 14:51, 17 October 2019
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	{{#ev:youtube\|https://youtu.be/GoDJGfqO3po\|640\|center}}		{{#ev:youtube\|https://youtu.be/GoDJGfqO3po\|640\|center}}
		+
		+	=== Relay Baseplating ===
		+	If using a relay lens for deep imaging, you have 2 options for baseplating. First, you can secure the large objective GRIN lens into the Miniscope and baseplate as you would with a single lens (see video above). Alternatively, you can cement that objective lens onto the animals head in order to make it more stable across imaging sessions. We have found this to be far better for stable recording across weeks. The video below steps through this option and demonstrates how to secure the objective GRIN lens into place while imaging.
		+
		+	{{#ev:youtube\|https://youtu.be/b-LrnOBX3j8\|640\|center}}

← Older revision		Revision as of 01:04, 16 November 2016
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	Apply dental cement to the anterior edge of the baseplate and fill in the gaps between the baseplate and skull on the animal’s left side. Allow the dental cement to dry and attach the plastic cap onto the top of the baseplate with a set screw.		Apply dental cement to the anterior edge of the baseplate and fill in the gaps between the baseplate and skull on the animal’s left side. Allow the dental cement to dry and attach the plastic cap onto the top of the baseplate with a set screw.

−	https://youtu.be/GoDJGfqO3po	+	{{#ev:youtube\|https://youtu.be/GoDJGfqO3po\|640\|center}}

← Older revision		Revision as of 01:03, 16 November 2016
Line 77:		Line 77:

	Apply dental cement to the anterior edge of the baseplate and fill in the gaps between the baseplate and skull on the animal’s left side. Allow the dental cement to dry and attach the plastic cap onto the top of the baseplate with a set screw.		Apply dental cement to the anterior edge of the baseplate and fill in the gaps between the baseplate and skull on the animal’s left side. Allow the dental cement to dry and attach the plastic cap onto the top of the baseplate with a set screw.
		+
		+	https://youtu.be/GoDJGfqO3po

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 Once the baseplate has dried, score the bottom and the sides of the baseplate using a dental drill to allow the dental glue to adhere the baseplate and skull well.
 {{#ev:youtube|https://youtu.be/z_32O2XoYI4|640|center}}
 === Cap Preparation ===

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 === Basic Equipment needed ===
 [[File:bp1.png|right|200px|thumb|Put a stack of magnets on a reference Miniscope and mark the top magnet to keep track of its polarity]]
 [[File:bp2.png|right|200px|thumb|Magnetically attach the top magnet to screwdriver, coat with cyanoacrylate glue on the edge, and push through hole on baseplate]]
@@ Line 55: / Line 52: @@
 [[File:bp4.png|right|200px|thumb|Coat bottom of baseplate with cyanoacrylate glue]]
 [[File:bp5.png|right|200px|thumb|Score bottom and edges of baseplate with a dental drill]]
 === Baseplate Preparation ===

@@ Line 50: / Line 50: @@
 You will also need cyanoacrylate glue, fine forceps, double distilled water, lens paper, and dental cement
 === Baseplate Preparation ===
 Before affixing the baseplate on an animal, you will need to prepare the baseplate.